ISBN: 9781617379390
Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality o… Altro …
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2011, ISBN: 1617379395
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2011, ISBN: 9781617379390
Methods and Protocols, Buch, Softcover, Softcover reprint of hardcover 1st ed. 2009, [PU: Humana Press Inc.], Humana Press Inc., 2011
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ISBN: 9781617379390
Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality o… Altro …
Sokolowski, Bernd (Herausgeber):
Auditory and Vestibular Research Methods and Protocols - edizione con copertina flessibile2011, ISBN: 1617379395
edizione con copertina rigida
Softcover reprint of hardcover 1st ed. 2009 Kartoniert / Broschiert Physiologie, Pathologie, Neurologie und klinische Neurophysiologie, Genetik (nicht-medizinisch), Cells; gene expressi… Altro …
2011
ISBN: 1617379395
edizione con copertina rigida
Softcover reprint of hardcover 1st ed. 2009 Kartoniert / Broschiert Physiologie, Pathologie, Neurologie und klinische Neurophysiologie, Genetik (nicht-medizinisch), molecularbiology; ph… Altro …
2011, ISBN: 9781617379390
Methods and Protocols, Buch, Softcover, Softcover reprint of hardcover 1st ed. 2009, [PU: Humana Press Inc.], Humana Press Inc., 2011
ISBN: 9781617379390
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Informazioni dettagliate del libro - Auditory and Vestibular Research
EAN (ISBN-13): 9781617379390
ISBN (ISBN-10): 1617379395
Copertina rigida
Copertina flessibile
Anno di pubblicazione: 2011
Editore: Humana Press Inc.
440 Pagine
Lingua: eng/Englisch
Libro nella banca dati dal 2011-09-04T14:46:15+02:00 (Zurich)
Pagina di dettaglio ultima modifica in 2024-03-26T20:36:20+01:00 (Zurich)
ISBN/EAN: 1617379395
ISBN - Stili di scrittura alternativi:
1-61737-939-5, 978-1-61737-939-0
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : sokolowski, sokol, sokolow, bernd
Dati dell'editore
Autore: Bernd Sokolowski
Titolo: Methods in Molecular Biology; Auditory and Vestibular Research - Methods and Protocols
Editore: Humana; Humana Press
428 Pagine
Anno di pubblicazione: 2011-01-20
Totowa; NJ; US
Stampato / Fatto in
Lingua: Inglese
155,14 € (DE)
159,49 € (AT)
171,50 CHF (CH)
POD
XII, 428 p. 61 illus., 13 illus. in color.
BC; Hardcover, Softcover / Biologie; Physiologie; Verstehen; cells; gene expression; gene transfer; genes; hybridization; microscopy; molecular biology; physiology; regulation; tissue; Human Physiology; Neurology; Biomedical Research; Pathology; Molecular Genetics; Neurologie und klinische Neurophysiologie; Medizinische Forschung; Pathologie; Genetik (nicht-medizinisch); BB
Nucleic Acid Protocols.- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning.- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays.- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections.- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification.- Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus.- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research.- Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium.- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear.- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear.- Molecular Biology of Vestibular Schwannomas.- Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens.- Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa.- Positional Cloning of Deafness Genes.- Amino Acid Protocols.- Twist-Off Purification of Hair Bundles.- Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System.- The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea.- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis.- In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation.- Identification of Transcription Factor–DNA Interactions Using Chromatin Immunoprecipitation Assays.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins inInner-Ear Sensory Epithelia.- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Imaging Protocols.- Fluorescence Microscopy Methods in the Study of Protein Structure and Function.- Ion Imaging in the Cochlear Hair Cells.- Atomic Force Microscopy in Studies of the Cochlea.Lists all the reagents, step-by-step techniques, and limitations for reproducible results in DNA/RNA tracing, protein identification, and microscopic imaging Clearly describes the protocols necessary for in vivo and in vitro gene delivery techniques Provides the tools to identify and isolate cochlear stem cells for the treatment of inner ear pathologies Includes lineage and fate mapping techniques for peripheral and central studies of auditory/vestibular development Describes techniques for identification and verification of protein-protein interactions Provides important tools for the basic and clinical researcher to identify genes and microbes underlying diseases of the ear including the cloning of deafness genes Includes supplementary material: sn.pub/extras
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