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David J. Sharp:

Mitosis - nuovo libro

2014, ISBN: 9781493903290

Methods and Protocols, eBooks, eBook Download (EPUB), Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis researc… Altro …

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Mitosis - nuovo libro

ISBN: 9781493903290

Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in is… Altro …

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David J. Sharp:
Mitosis - nuovo libro

ISBN: 9781493903290

Life Sciences; Cell Biology; Cell Cycle Analysis; Gene Expression cell division, chromosome segregation, complex cell free extracts, microtubule based machine, microtubule cytoskeleton in… Altro …

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EAN (ISBN-13): 9781493903290
Anno di pubblicazione: 2014
Editore: Springer New York

Libro nella banca dati dal 2018-02-04T11:12:54+01:00 (Zurich)
Pagina di dettaglio ultima modifica in 2024-03-16T22:55:32+01:00 (Zurich)
ISBN/EAN: 9781493903290

ISBN - Stili di scrittura alternativi:
978-1-4939-0329-0


Dati dell'editore

Autore: David J. Sharp
Titolo: Methods in Molecular Biology; Mitosis - Methods and Protocols
Editore: Humana; Springer US
308 Pagine
Anno di pubblicazione: 2014-03-14
New York; NY; US
Stampato / Fatto in
Lingua: Inglese
106,99 € (DE)
110,00 € (AT)
124,00 CHF (CH)
Available
X, 308 p. 83 illus., 71 illus. in color.

EA; E107; eBook; Nonbooks, PBS / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; cell division; chromosome segregation; complex cell free extracts; microtubule based machine; microtubule cytoskeleton in cells; microtubule structure in cells; microtubule-associated proteins; microtubules; mitosis; mitotic defects; quantifying aneuploidy; spindle apparatus; Cell Cycle Analysis; gene expression; B; Cell Biology; Cell Cycle Analysis; Gene Expression; Cell Biology; Cytological Techniques; Molecular Genetics; Biomedical and Life Sciences; Wissenschaftliche Ausstattung, Experimente und Techniken; Genetik (nicht-medizinisch); BB

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.

Mitosis: Methods and Protocols Methods in Molecular Biology Mitosis: Methods and Protocols

Part I: Imaging and Measurement of Mitotic Spindle Dynamics In Vivo

Embryos by Live Cell Imaging and Quantitative Modeling

Ingrid Brust-Mascher, Gul Civelekoglu-Scholey, and Jonathan M. Scholey

 

2. Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells

Justin Decarreau, Jonathan Driver, Charles Asbury and Linda Wordeman

 

Embryos

Daniel Needleman

 

4. Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells

Takashi Murata and Tobias I. Baskin

 

5. Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics

Ge Yang

 

 

Part II: Analysis and Manipulation of the Microtubule Cytoskeleton in Cells and In Vitro

Cells for Studying the Microtubule Cytoskeleton

Jonathan Nye, Daniel W. Buster, and Gregory C. Rogers

 

Embryos

Justus Tegha-Dunghu, Eva M. Gusnowski, and Martin Srayko

 

 Egg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators 

Eliza Żyłkiewicz and   P. Todd Stukenberg

 

 

Part III: Specialized Biophysical and Microscopy Approaches for Analyzing Microtubules and Microtubule Associated Proteins

 

9. Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays

Matthew P. Nicholas, Lu Rao, and Arne Gennerich

 

10. An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules

Matthew P. Nicholas, Lu Rao, and Arne Gennerich

 

11. Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins

Sebastian P. Maurer, Franck J. Fourniol, Andreas Hoenger, and Thomas Surrey

 

12. The Segmentation of Microtubules in Electron Tomograms using Amira

Stefanie Redemann, Britta Weber, Marit Möller, Jean-Marc Verbavatz, Anthony A. Hyman, Daniel Baum, Steffen Prohaska, and Thomas Müller-Reichert

 

 

Part IV: Extras

13. Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers

and Nicolas Minc

 

14. Four- color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells

Francesca Faggioli, Jan Vijg,  and Cristina Montagna

Drosophila C. elegans Drosophila C. elegans Xenopus

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

seeks to provide diverse methods and  new techniques to address new or old questions related to the mechanisms of mitosis.

Mitosis: Methods and Protocols Methods in Molecular Biology Mitosis: Methods and Protocols

Presents current up-to-date methodologies

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras



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