Exocytosis and Endocytosis - edizione con copertina flessibile

Libro nella banca dati dal 2016-11-30T17:07:37+01:00 (Zurich)
Pagina di dettaglio ultima modifica in 2023-11-12T10:40:15+01:00 (Zurich)
ISBN/EAN: 9781493946945

ISBN - Stili di scrittura alternativi:
1-4939-4694-3, 978-1-4939-4694-5
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : ivanov andrei

Dati dell'editore

Autore: Andrei I. Ivanov
Titolo: Methods in Molecular Biology; Exocytosis and Endocytosis
Editore: Humana; Springer US
435 Pagine
Anno di pubblicazione: 2016-08-23
New York; NY; US
Stampato / Fatto in
Lingua: Inglese
117,69 € (DE)
120,99 € (AT)
130,00 CHF (CH)
POD
XIII, 435 p. 93 illus., 55 illus. in color.

BC; Hardcover, Softcover / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; biochemical assays; cultured cells; endocytosis; exocytosis; live cell imaging; vesicular trafficking; Cell Biology; BB

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

Pharmacological Inhibitors of Exocytosis and Endocytosis:  Novel Bullets for Old Targets.- Systematic Analysis of Endocytosis by Cellular Perturbations.- Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System.- Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides.- Secretion of Circular Proteins Using Sortase.- Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation.- Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.- Probabilistic Density Maps to Study the Spatial Organization of Endocytosis.- Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors.- Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells.- SNAP-Tag to Monitor Trafficking of  Membrane Proteins in Polarized Epithelial Cells.- FLAsH-PALM:  Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling.- Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers.- Synchronization of Secretory Cargo Trafficking in Populations of Cells.- Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins.- A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering.- A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays.- Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy.- Nanocones to Study Initial Steps of Endocytosis.- A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography.- VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy.- Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using theEndomembrane Trafficking Network.- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans.- Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers.- Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling.- Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms.- The “In Situ” Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues.- Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy.- Measurement of Dynamic F-actin Changes During Exocytosis.

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.