2D PAGE: Sample Preparation and Fractionation Volume 1 - copertina rigida, flessible

EAN (ISBN-13): 9781588297228
ISBN (ISBN-10): 1588297225
Copertina rigida
Copertina flessibile
Anno di pubblicazione: 2008
Editore: Humana Press Inc.
459 Pagine
Peso: 1,043 kg
Lingua: eng/Englisch

Libro nella banca dati dal 2007-04-28T03:44:20+02:00 (Zurich)
Pagina di dettaglio ultima modifica in 2024-04-17T10:31:01+02:00 (Zurich)
ISBN/EAN: 9781588297228

ISBN - Stili di scrittura alternativi:
1-58829-722-5, 978-1-58829-722-8
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : anton, sch, posch, john murphy, pösch
Titolo del libro: volume, page, sample preparation

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Dati dell'editore

Autore: Anton Posch
Titolo: Methods in Molecular Biology; 2D PAGE: Sample Preparation and Fractionation - Volume 1
Editore: Humana; Humana Press
459 Pagine
Anno di pubblicazione: 2008-03-19
Totowa; NJ; US
Lingua: Inglese
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
Available
XIII, 459 p. 133 illus., 2 illus. in color.

BB; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Amino acid; Escherichia coli; Lipid; Organelle; Polyacrylamid-Gelelektrophorese; Proteomics; biochemistry; biomedicine; enzymes; glycoprotein; membrane proteins; polymer; protein; protein complexes; proteins; Protein Biochemistry; Cell Biology; Biochemistry; Microbial Genetics; Zellbiologie (Zytologie); Mikrobiologie (nicht-medizinisch); Genetik (nicht-medizinisch); BC

Sample Preparation Basics.- Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.- Bacteria and Yeast Cell Disruption Using Lytic Enzymes.- Sample Solublization Buffers for Two-Dimensional Electrophoresis.- Quantitation of Protein in Samples Prepared for 2-D Electrophoresis.- Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis.- Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction.- Protein Labeling Techniques.- Difference Gel Electrophoresis Based on Lys/Cys Tagging.- Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine.- Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC).- ICPL—Isotope-Coded Protein Label.- Radiolabeling for Two-Dimensional Gel Analysis.- Fractionation of Proteins by Chemical Reagents and Chromatography.- Sequential Extraction of Proteins by Chemical Reagents.- Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg.- Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis.- Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns.- Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures.- Fractionation of Proteins by Immobilized Metal Affinity Chromatography.- Fractionation of Proteins by Heparin Chromatography.- Fractionation of Proteins by Electrophoresis Methods.- Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric Focusing.- Microscale Isoelectric Focusing in Solution.- Prefractionation, Enrichment, Desalting and Depleting of Low Volume and Low Abundance Proteins and Peptides Using the MF10.-Sample Prefractionation in Granulated Sephadex IEF Gels.- Free-Flow Electrophoresis System for Plasma Proteomic Applications.- Protein Fractionation by Preparative Electrophoresis.- Enrichment Strategies for Organelles, Multiprotein Complexes, and Specific Protein Classes.- Isolation of Endocitic Organelles by Density Gradient Centrifugation.- Isolation of Highly Pure Rat Liver Mitochondria with the Aid of Zone-Electrophoresis in a Free Flow Device (ZE-FFE).- Isolation of Proteins and Protein Complexes by Immunoprecipitation.- Isolation of Phosphoproteins.- Glycoprotein Enrichment Through Lectin Affinity Techniques.- Isolation of Bacterial Cell Membranes Proteins Using Carbonate Extraction.- Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems.- The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis.- Isolation of Membrane Protein Complexes by Blue Native Electrophoresis.- Tissue Microdissection.
Contains step-by-step instructions that ensure successful results Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls