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2008, ISBN: 9781588299123

Editor: Vincan, Elizabeth, Humana, Hardcover, Auflage: 2008, 336 Seiten, Publiziert: 2008-10-30T00:00:01Z, Produktgruppe: Book, 0.95 kg, Verkaufsrang: 10647819, Biochemistry, Biological S… Altro …

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WNT Signalling: Preliminary Entry 2158: Preliminary Entry 2158 v. 1 (Methods in Molecular Biology): Volume 1: Pathway Methods and Mammalian Models: 468 - copertina rigida, flessible

2008, ISBN: 9781588299123

Editor: Vincan, Elizabeth, Humana, Hardcover, Auflage: 2008, 336 Seiten, Publiziert: 2008-10-30T00:00:01Z, Produktgruppe: Book, 0.95 kg, Verkaufsrang: 10647819, Biochemistry, Biological S… Altro …

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ISBN: 9781588299123

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Wnt Signaling - copertina rigida, flessible

2008, ISBN: 9781588299123

Volume 1: Pathway Methods and Mammalian Models, Buch, Hardcover, 2008 ed. [PU: Humana Press Inc.], Humana Press Inc., 2008

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Wnt Signaling: Volume 1: Pathway Methods and Mammalian Models (Methods in Molecular Biology, 468, Band 468)

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource.

Informazioni dettagliate del libro - Wnt Signaling: Volume 1: Pathway Methods and Mammalian Models (Methods in Molecular Biology, 468, Band 468)


EAN (ISBN-13): 9781588299123
ISBN (ISBN-10): 1588299120
Copertina rigida
Anno di pubblicazione: 2008
Editore: Vincan, Elizabeth, Humana
333 Pagine
Peso: 0,839 kg
Lingua: eng/Englisch

Libro nella banca dati dal 2009-03-31T17:10:31+02:00 (Zurich)
Pagina di dettaglio ultima modifica in 2024-02-25T16:30:10+01:00 (Zurich)
ISBN/EAN: 9781588299123

ISBN - Stili di scrittura alternativi:
1-58829-912-0, 978-1-58829-912-3
Stili di scrittura alternativi e concetti di ricerca simili:
Titolo del libro: signalling, wnt pathway, wnt signaling, mammalian biology, 2158, 468


Dati dell'editore

Autore: Elizabeth Vincan
Titolo: Methods in Molecular Biology; Wnt Signaling - Volume 1: Pathway Methods and Mammalian Models
Editore: Humana; Humana Press
336 Pagine
Anno di pubblicazione: 2008-10-09
Totowa; NJ; US
Stampato / Fatto in
Peso: 0,954 kg
Lingua: Inglese
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
XVI, 336 p. 76 illus., 2 illus. in color.

BB; Cell Biology; Hardcover, Softcover / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; Activation; Calcium; Canonical Wnt/FZD signaling; Degenerative diseases and cancer; Embryogenesis; Non-canonical signaling; Promoter; Protein-protein interaction; Tissue homeostasis; Wnt/FZD function; genes; molecular biology; proteins; tissue; transcription; Cell Biology; BC; EA

Part I. Wnt signaling pathway methods A: Canonical Wnt/FZD signaling Chapter 1. The Canonical Wnt/b -Catenin Signaling Pathway. Nick Barker Chapter 2. Isolation and application of bioactive Wnt proteins. Karl Willert Chapter 3: Purification and Wnt inhibitory activity of secreted Frizzled-related proteins. Vladimir Wolf, Yoshimi Endo and Jeffery S Rubin Chapter 4. Measuring GSK3 expression and activity in cells. Adam R Cole and Calum Sutherland Chapter 5.Inhibition of Glycogen Synthase Kinase-3. Andrei V Ougolkov and Daniel D Billadeau Chapter 6. Detection of cytoplasmic and nuclear localization of Adenomatous Polyposis Coli (APC) protein in cells. Mariana Brocardo and Beric R Henderson Chapter 7. Detection of b -catenin localization by immunohistochemistry. Nick Barker and Maaike van den Born Chapter 8. Assaying b -catenin/TCF transcription with b -catenin/TCF transcriptional-based reporter constructs. Travis L Biechele and Randall T Moon Chapter 9. Native promoter reporters validate transcriptional targets. Otto Schmalhofer, Simone Spaderna and Thomas Brabletz B: Non-canonical Signaling Chapter 10. b -Catenin-independent Wnt pathways: signals, core protein and effectors. Richard G James, William H Conrad and Randall T Moon Chapter 11. Image Analysis of Calcium Release Dynamics. Christina M Freisinger, Douglas W Houston and Diane Slusarski Chapter 12. Detecting PKC phosphorylation as part of the Wnt/calcium pathway in cutaneous melanoma. Samudra K Dissanayake and Ashani Weeraratna Chapter 13. Measuring CamK II activity in Xenopus embryos as a read-out for non-canonical Wnt signaling. Michael Kuhl and Petra Pandur Chapter 14. Analysis of Wnt7a-stimulated JNK activity and cJUN Phosphorylation in Non-Small Cell Lung Cancer Cells. Lynn E Heasley and Robert A Winn Chapter 15. ROCK enzymatic assay. John D Doran and Marc D Jacobs Chapter 16. Detection of Planar polarity proteins in mammalian cochlea. Mireille Montcouquiol and Nathalie Sans Part II. Mammalian Model systems for Wnt/FZD function Chapter 17. Proteomic analyses of protein complexes in the Wnt pathway. Stephane Angers Chapter 18. In situ hybridization to evaluate the expression of Wnt and Frizzled genes in mammalian tissues. Kestutis Planutis, Marina Planutiene and Randall F Holcombe Chapter 19. Assaying Wnt5a-mediated invasion in melanoma cells. Michael P. O’Connell, Amanda D. French, Poloko D. Leotlela and Ashani T. Weeraratna Chapter 20. Co-culture methodologies for the study of Wnt signals. Kestutis Planutis, Marina Planutiene and Randall F Holcombe Chapter 21. Analysis of Wnt/FZD-mediated signaling in a cell line model of colorectal cancer morphogenesis. Elizabeth Vincan, Robert H Whitehead and Maree C Faux Chapter 22. Analyzing tissue and gene function in intestinal organ culture. Helen E Abud. Heather M Young and Donald F Newgreen Chapter 23. The Genetics of Wnt Signaling During Early Mammalian Development. Terry Yamaguchi Chapter 24. Tissue-specific Transgenic, Conditional Knock-out and Knock-in Mice of Genes in the Canonical Wnt signaling Pathway. Koji Aoki and Makoto M Taketo

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