Mycobacteria Protocols - copertina rigida, flessible

EAN (ISBN-13): 9781588298898
ISBN (ISBN-10): 1588298892
Copertina rigida
Anno di pubblicazione: 2008
Editore: Humana Press Inc.
456 Pagine
Peso: 0,794 kg
Lingua: eng/Englisch

Libro nella banca dati dal 2008-12-13T07:31:37+01:00 (Zurich)
Pagina di dettaglio ultima modifica in 2022-07-17T18:30:40+02:00 (Zurich)
ISBN/EAN: 9781588298898

ISBN - Stili di scrittura alternativi:
1-58829-889-2, 978-1-58829-889-8
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : parish, tan, claire, brown, amanda
Titolo del libro: mycobacteria, 465, textbook surgery

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Dati dell'editore

Autore: Tanya Parish; Amanda Claire Brown
Titolo: Methods in Molecular Biology; Mycobacteria Protocols
Editore: Humana; Humana Press
456 Pagine
Anno di pubblicazione: 2008-10-20
Totowa; NJ; US
Lingua: Inglese
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
Available
XIX, 456 p.

BB; Hardcover, Softcover / Biologie/Mikrobiologie; Mikrobiologie (nicht-medizinisch); Verstehen; DNA; Human and animal pathogens; Immunodeficiency; Microarray; Opportunistic infections; Promoter; Proteomics; Strain typing; Sub-cellular fractionation; Tuberculosis; bacteria; biosynthesis; gene expression; genes; molecular biology; Bacteria; Microbiology; Biochemistry; Biomedical Research; Biochemie; Medizinische Forschung; BC; EA; BC

Isolation of Mycobacterium Species Genomic DNA.- Isolation of Mycobacterial RNA.- Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization.- Analyzing Lipid Metabolism: Activation and ?-Oxidation of Fatty Acids.- Analysis of Lipid Biosynthesis and Location.- Whole Genome Analysis Using Microarrays.- Use of DNA Microarrays to Study Global Patterns of Gene Expression.- Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria.- Transport Assays and Permeability in Pathogenic Mycobacteria.- Continuous Culture of Mycobacteria.- Measuring Minimum Inhibitory Concentrations in Mycobacteria.- Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts.- Electroporation of Mycobacteria.- Ins and Outs of Mycobacterial Plasmids.- The Use of Temperature-Sensitive Plasmids in Mycobacteria.- Heterologous Expression of Genes in Mycobacteria.- Inducible Expression Systems for Mycobacteria.- Assaying Promoter Activity Using LacZ and GFP as Reporters.- Construction of Unmarked Deletion Mutants in Mycobacteria.- Construction of Targeted Mycobacterial Mutants by Homologous Recombination.- Phage Transposon Mutagenesis.- Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction.- Gene Switching and Essentiality Testing.- Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis.- Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis.- Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray.- MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms.- Environmental Amoebae and Mycobacterial Pathogenesis.
Fully updated from the first edition to offer cutting edge methods for manipulation of Mycobacteria Includes methods for preparation and analysis of sub-cellular fractions Provides description of genetic systems and instructions for manipulating the genome Contains techniques for studying gene expression, singly and globally, and updated techniques for mutagenesis and gene essentiality testing With detailed protocols for genomic, transcriptomic and proteomic analysis, and new molecular methods for typing and drug susceptibility profiling Includes supplementary material: sn.pub/extras